Sharpshooter Calamity Jane takes it upon herself to recruit a famous actress and bring her back to the local saloon, but jealousy soon gets in the way.
Al Stewart and Wilbert are magicians doing a stage act when they run into Wilbert's cousin, Dorothy McCoy. They find out that Wilbert's grandfather, Squeeze-box McCoy, had treasure hidden in the hills of Kentucky, which they go to find.
Sailor meets singer in Cuba. He's due to ship out, but hits it off with her, so he sticks around. Trouble brewing on many fronts - singer's best friend doesn't like the sailor, and singer has another suitor anyway. Rich guy takes her to all the fancy Cuban sports events - jai-alai, horse racing. Much more glamorous than spending time with Sailor. Can he win her over before he gets in trouble for letting his ship sail without him?
An illiterate stooge in a traveling medicine show wanders into a strange town and is picked up on a vagrancy charge. The town's corrupt officials mistake him for the inspector general whom they think is traveling in disguise. Fearing he will discover they've been pocketing tax money, they make several bungled attempts to kill him.
When asked about the Ghost Riders song he sings, Gene Autry tells this legend: Gene is about to resign as an investigator for the county attorney and go into the cattle business with his pal Chuckawalla Jones but decides instead to help Anne Lawson clear her father, rancher Ralph Lawson, of a false murder charge. He looks for the three witnesses who can testify that Lawson shot only in self defense in killing a gambler, but the witnesses are terrorized by another gambler, town boss Rock McCleary, who shoots witness Pop Roberts Morgan. Fatally wounded, Pop gives Gene the information needed to clear Lawson, then dies crying the "Ghost Riders" are coming for him. Gene then heads for a showdown with McCleary.
While trailing Forest Ranger Charles Carter, who is suspected of permitting lumber man Henry Mitchell to cut restricted timber, Gene fires at a dangerous mountain lion and apparently kills Carter. Actually, Bill Wright, Mitchell's associate, killed Carter because the ranger had discovered tussock moth infestation in the forest, and if the infestation was not reported, the trees would die and have to be cut, thereby profiting Mitchell and Wright. In order to compensate the best he can, Gene sells his sportsman's camp and gives the money to Carter's daughter Helen . En route to Texas, Gene discovers the infestation and is assigned by the Forest Department to supervise the program of spraying the area with DDT from the air. After the first day of spraying, the DDT is blamed by furious stock men for the many animals found dead of poisoning.
Former burlesque star May and her daughter Peggy dance in the chorus. When May has a fight with featured dancer Bubbles, Bubbles leaves the show and Peggy takes her place. When Peggy falls in love with wealthy Randy, May fears class differences may lead to misery.