Singing cowboy Eddie Dean and his sidekick Soapy (Roscoe Ates) enter into the thick of things when they thwart a stagecoach holdup. Our heroes take it upon themselves to champion the cause of stage-line owner Margie Rodgers (Helen Mowery), who's being victimized by an unknown villain. Dean suspects that there's more to the case than mere robbery, and he's right: someone wants to gain control of Margie's business, and that someone is?
Caxton has broken out of prison and Eddie has been sent to bring him in. Caxton is known by the polka dot band on his hat and Eddie has Soapy wear one like it. This gets Soapy arrested as soon as he rides into town but it leads Eddie to Varney and he realizes Varney will lead him to Caxton.
Eddie and his sidekicks have been called in to help get a new telegraph line through. Dawson and his men along with his stooge Judge are out to stop them. When Eddie and the boys catch three of Dawson's men destroying telegraph equipment, the Judge releases them and this leads to the showdown between the two sides.
Eddie Dean is a Cattlemen's Association agent investigating a serious rash of rustlings along with sidekicks Soapy (Roscoe Ates) and Waco (Lee Bennett. The latter bears a striking resemblance to Lawrence ranch foreman Bert Ford (also Bennett), who has been the target of several assassination attempts. Rancher Lawrence (Lee Roberts) and Eddie decide that Waco shall impersonate Ford, who is hiding out in a hotel room.
After being shanghaied in a plot to have him declared dead and take over his ranchero and other properties, Don Ricardo, disguised as a peon, returns to Old California, and begins the fight to reclaim what is his.
Eddie Dean (Eddie Dean) and his partner Soapy Jones (Roscoe Ates), under government orders, proceed to the ranch of J.C. Morgan (Shirley Patterson to buy cavalry remounts for the Army. At the ranch, they find out that J.C. is a girl. The nearby town of Dow City is under the control of a lawless trio headed by Trigger (Lee Roberts, Clem Kensington (Foxy Callahan) and Joe Morino (Dennis Moore). A member of the gang is Tucson Brown (Lee Bennett), one of J.C.'s trusted hands. When Eddie decides to buy the horses, Tucson steals the herd to prevent the sale. Soldiers, sent to investigate, are brutally murdered. The aroused townspeople elect Tennessee (William Fawcett, J.C.'s foreman, as sheriff. When the outlaws murder Tennessee, Eddie and Soapy, along with the reformed Tucson, swing into action.
When an agricultural professor returns home to the farm with her scientifically-raised mule for a needed rest, they find themselves caught up in a movie being filmed in the Ozarks.
Eddie Dean's assignment is to thwart the efforts of a crooked gambler, Brad Barton, to take over the property of his half-brother Bill Ryan. In order to secure the ranch, which is believed to hold large silver deposits, the scheming relative contracts to have Ryan killed. He then presents a forged will to the court naming himself as the sole heir. Shocked by the tide of events, Ryan's two rightful heirs, his grown daughter Robin and young son "Freckles" are determined to remain on their father's property. Eddie and his sidekick, Soapy Jones, arrive on the scene in time to enter the fight on the side of Robin and "Freckles."
Medicine show proprietor Doc Lattimer has in his possession a map showing the location of a cache of stolen gold. His son Don favors keeping the gold rather than returning it to the express company to which it rightfully belongs, and steals the map, only to find himself menaced by outlaw leader Cal and his gang.
An investigator from the War Crimes Commission travels to Connecticut to find an infamous Nazi, who may be hiding out in a small town in the guise of a distinguished professor engaged to the Supreme Court Justice’s daughter.
Duke Dillon has his gang robbing stagecoaches carrying gold which is then melted down by his father. But Eddie and his sidekick Soapy are on the job and they are aided by undercover man Nevada.
Dawson is running rustled cattle across the abandoned Trenton ranch and has given it the reputation of a ghost ranch to keep people away. When Henty Tenton arrives from England to take over the ranch, Dawson tries to get rid of him. But Billy and Fuzzy are on hand to help Henry and it's not long before they have to go into action.
A convicted thief in Dartmoor prison hides the location of the stolen Bank of England printing plates inside three music boxes. When the innocent purchasers of the boxes start to be murdered, Holmes and Watson investigate.
While Fuzzy is waiting for his mail order bride, McCallister frames him for murder. McCallister then advises Matilda to marry Fuzzy so she will get the money when he is hung. But Billy breaks up the wedding and goes after the real killer.
The happy Indians live in Antelope Valley and Eddie is the new Indian Agent. Everything seems fine until the town selectmen want the valley occupied by the Indians because it contains silver. So they hire outlaw Indians and Chico to start trouble hoping that the army will forcibly remove them from the valley and they will claim it. But Father Sullivan and Eddie believe the Indians are being wronged even though they cannot convince anyone else.
One of four western films made for PRC by bantam-weight Bob Steele, Ambush Trail stars Steele as cowpoke Curley Thompson. The villain of the piece intends to bankrupt all the local ranchers and grab up the surrounding property for himself. But with Curley involved, the bad guy and his minions don't have a chance. The screenplay, by D. W. Griffith alumnus Elmer Clifton, is a medley of western cliches, pausing every so often for a first-rate action sequence. Perennial sagebrush sidekick Sid Saylor provides negligible comedy relief.