As foreman of a dude ranch, Gene has two problems. One is a guest, the spoiled daughter of a millioniare, and the other is the disgruntled ex-foreman that Gene replaced, now just a ranch hand. Gene eventually gets the daughter straightened out but has to fire the ex-foreman and this leads to trouble when he returns intent on revenge.
Cholita, after a long absence in Mexico City, is returning home to take up her duties as head of the rancho and, as everyone expects, to marry her childhood sweetheart José. Expectations are somewhat dashed as she shows up with Fernando to whom she is engaged. This makes José and Cholita's uncle more than a little bit put out as Fernando is not only not a Mexican, he is also a city slicker afraid of the country.
To fight a poisonous weed, ranchers are burning their land. Gene is the Inspector brought in and he recommends spraying. The spraying goes well until the Larabee ranch is reached. When Larrabee refuses to allow the equipment on his land, Gene has it sprayed by airplane. Cattle must stay off recently sprayed land and when a Larrabee man shoots down the plane, the crash sends the cattle stampeding toward the newly sprayed land.
Fraternity brothers enter one of their own into a scholarship lottery after a women's college insults them. Though the Zeta boys are celebrated for their comedy drag revue, staying undercover as a woman at an all-girls' school wasn't part of the rehearsal!
When Jesse learns that Krager is cheating settlers, he and his gang rob trains to obtain money for them to purchase their land. Krager, finding a Jesse look alike in Burns, hires him to wreck havoc on the ranchers. When Jesse kills Burns he switches clothes and goes after the culprits.
Rawley University is about to receive a star athlete who could give it the first championship rowing team it's ever had. Unfortunately, he gets drafted into the army before he's able to join the team. Two of the team's members get the bright idea of passing off a burly truck driver as the "athlete". Complications ensue.
Razz accidentally shoots his wife Martha when his hunting rifle drops on the floor and discharges. The church congregation gathers at Martha’s bedside to pray for her recovery, and during this period an angel arrives to take Martha’s spirit from her body, but she is tempted by the slick Judas Green, who is an agent for Satan.
Jimmy, the owner of a failed music shop, goes to work with his uncle, the owner of a food factory. Before he gets there, he befriends an Irish family who happens to be his uncle's worst enemy because of their love for music and in-house band who constantly practices. Soon, Jimmy finds himself trying to help the band by getting them gigs and trying to reconcile the family with his uncle.
Tom Cameron, aka the Lone Rider, and his faithful sidekick, Fuzzy Jones, flee across the Rio Grande to avoid assassination by crooked lawman Deputy Hatfield, only to have the Mexican cops accuse Cameron of being the notorious bandit El Puma. At Hatfield's behest, they are also accused of kidnapping the local mayor's son, and now the pair must prove their innocence and find a way to stop Hatfield's lawless ways.
Rich playboy Drogo Gaines is in imminent danger of marrying a gold digger, and escapes by feigning insanity. The joke's on him when he wakes up in an asylum full of comical lunatics. There he befriends Colonel Carraway, and together they escape, catching a ride with a beautiful blonde who proves to be Penguin Moore, carnival owner.
Perky young Nanette attempts to save the marriage of her uncle and aunt by untangling Uncle Jimmy from several innocent but ensnaring flirtations. Attempting one such unentanglement, Nanette enlists the help of theatrical producer Bill Trainor, who promptly falls in love with her. The same thing happens when artist Tom Gillespie is called on for help. But soon Uncle Jimmy's flirtations become too numerous, and Nanette's romances with Tom and Bill run into trouble. Will Uncle Jimmy's marriage survive, and will Nanette find happiness with Tom, Bill, or somebody else?
Storm is out to wreck Ace's stage line. When Tex arrives to help Ace, Storm brings in hired killer Mule Bates. But Tex and Bates know each other and the two devise a plan to fool Storm.
When Moreland's henchman attack Bob Horner the Range Busters break it up. Horner dies but they get the baby Moreland was after. He must have it to become it's guardian and take control of the Horner ranch. When the henchmen catch up with Alibi, they take the baby cradle not realizing Alibi has substituted his dummy Elmer. Crash having evidence Moreland is the one they want, now has a plan to expose him.
A none-too-popular (nor good) radio singer, Rita Wilson is murdered while singing on the air in a radio studio. Radio page boy, Frankie Ryan, and his janitor pal, Jeff, solve the mystery for the none-too-sharp police.
Gene Autry and sidekick Frog Millhouse depart Madison Square Garden and NYC heading west for home in their car and a horse trailer carrying Gene's horse, Champion. They discover that Ronnie Willoughby, a young boy just off the boat from school in England, has hitched a ride, thinking that Gene and Frog were sent by his father to meet him. Ronnie thinks his father is a big rancher in the west and doesn't know that his father, Alfred Willoughby, is serving time in San Quentin prison because of a frame-up by the officials of a packing company. To keep the father from testifying against them, the packing company officials, Carter, Jenkins and Martin, have arranged for the boy to be kidnapped. Along the way a runaway bride, Joyce Halloway, and her young sister Patsy join the troupe.
In and around some great blues, swing and jazz music, a very unpopular band-leader. Prince Ellis, is killed in a Harlem nightclub, and, in and around some more great music, a detective finds the lists of suspects is very long, as Prince Ellis was indeed very unpopular with many citizens.
After noted violinist Arthur Williams suffers a hand injury which ends his playing career, his hopes are transferred to his son, who prefers swing music to classical.
Rancher Autry takes a job singing on the radio to aid farmers and ranchers whose lands were destroyed by raging floods. Blaming crooked politicians, he goes to Washington and tries to put through a food control bill and finds he has a lot to learn. In this classic release, Gene introduces his immortal theme song, "Back in the Saddle Again," which has gone on to become a piece of American History.