When his well-meaning sidekick (Smiley Burnette) buys a cow farm instead of a cattle ranch, singing cowpoke Gene Autry prepares to embrace the dairy business. But with a corrupt association bent on driving up milk prices, it's up to newly elected Sheriff Gene to clean up the mess. Country music icon Patsy Montana sings "I Want to Be a Cowboy's Sweetheart," while radio crooners the Texas Rangers perform alongside Autry.
John Abbott returns to the desert land he owns, and after being wounded by hired gunman Chick Chance, he is befriended by rancher Andrew Naab and his son, Marvin. Naab's daughter, Marian, falls in love with John but is about to marry Snap Thornton to keep a promise made by her father. She runs away on her wedding day but is captured and held hostage by outlaw Henry Holderness. John, the Naabs and fellow ranchers rush to her rescue.
Cavanaugh and McCauley are after the ranchers land. When the Government announces the land will be put up for auction, the ranchers pool their money only to have it stolen by Cavanaugh's men. They then plan to sell their cattle but Cavanaugh announces a fake gold strike and the cowhands all leave. But Gene's hobo friend the Judge says he will get the cattle to market and he sends out a signal to his hobo friends.
A group of people traveling on a stagecoach find their journey complicated by the threat of Geronimo, and learn something about each other in the process.
When shifty cattlemen Belknap (Walter Miller) and H.R. Shelby (Gordon Hart) are caught shipping infected animals to Mexico, they frame inspector Gene Autry. Now Autry and his sidekick, Frog Millhouse (Smiley Burnette), must catch the bad guys in the act and set things straight. June Storey co-stars as rancher Martha Wheeler. Autry sings "I'm Gonna Round Up My Blues," "Moonlight on the Ranch House" and "Big Bull Frog."
Foxes are mysteriously disappearing from fox farms and Agent Don has been sent to investigate. Brad and Pete are the culprits and they are using a dog that can climb the security fence to steal the foxes. Don brings a dog with him and when Carol sees a dog stealing a fox, she accuses Don.
Knowing that is contains valuable helium gas, a gang of bad guys first tries to purchase the ranch which Gene straw-bosses. When that fails, they lay a hidden pipeline to snag the gas.
In a wild west community populated entirely by residents with dwarfism, the Larson and Preston familes have been in a generations-long feud, recently strained by the actions of cattle rustler and overall devious criminal Bat Haines. Buck Larson, the hero of our tale, takes on Haines, and tries to win the heart of Nancy Preston while resolving the tensions between his and her families.
Mary Smith decides after a lifetime of being a shut-in to do something wild while her father is out campaigning for the presidency, so she takes off for the family's home in West Palm Beach and inadvertently becomes romantically entangled with earnest cowboy Stretch Willoughby. Neither the dalliance nor the cowboy fit with the upper class image projected by her esteemed father, forcing her to choose.
Gene takes care of three tough kids sent west from Chicago after their father died and left them a cattle ranch. They help him catch a bunch of rustlers.
Saunders with his Cattlemen's Protective Agency is running roughshod over the ranchers. Lawyer Larry Kimball is fighting him but he needs a rancher that will stand up with him against Saunders. He finds him when Lou Gehrig retires from baseball to take up ranching. Lou expects to relax on his ranch but quickly joins Larry in the fight.
Young Englishman inherits ranch which he wants to sell, but Gene's gonna turn him into a real westerner instead. When new owner Spud arrives from England, Autry convinces him not to sell the ranch but to raise horses for the Army. When both Autry's and Neale's bids are the same, the Colonel calls for a race to decide the winner. But that night Neale has Autry's stable burned.
Gene and Frog, out to stop a bunch of cattle rustlers, assume the identities of what they believe to be dead bandits, which soon gets them in big trouble.
A crooked real estate manipulator sells worthless land on mortgage to flood refugees, then tries to profit by reselling the land to the state, committing murder in the process, as the Three Mesquiteers work to bring him and his gang to justice.