European bad guy Baron Bendor leads some local townsmen in a plot to obtain horses through theft. Hoppy and his sidekicks Lucky and Speedy must find and expose the horse thieves.
Roy is a Confederate officer stationed in Missouri during the Civil War. He must put an end to outlaw gangs working under the pretense of service to the Confederacy.
When his well-meaning sidekick (Smiley Burnette) buys a cow farm instead of a cattle ranch, singing cowpoke Gene Autry prepares to embrace the dairy business. But with a corrupt association bent on driving up milk prices, it's up to newly elected Sheriff Gene to clean up the mess. Country music icon Patsy Montana sings "I Want to Be a Cowboy's Sweetheart," while radio crooners the Texas Rangers perform alongside Autry.
John Abbott returns to the desert land he owns, and after being wounded by hired gunman Chick Chance, he is befriended by rancher Andrew Naab and his son, Marvin. Naab's daughter, Marian, falls in love with John but is about to marry Snap Thornton to keep a promise made by her father. She runs away on her wedding day but is captured and held hostage by outlaw Henry Holderness. John, the Naabs and fellow ranchers rush to her rescue.
Cavanaugh and McCauley are after the ranchers land. When the Government announces the land will be put up for auction, the ranchers pool their money only to have it stolen by Cavanaugh's men. They then plan to sell their cattle but Cavanaugh announces a fake gold strike and the cowhands all leave. But Gene's hobo friend the Judge says he will get the cattle to market and he sends out a signal to his hobo friends.
A group of people traveling on a stagecoach find their journey complicated by the threat of Geronimo, and learn something about each other in the process.
The Colonel sends Fred Dawson and Doc Flanders to investigate a cattleman sheepman war. Posing as a two man medicine show, they quickly become involved. When Fred tries to bring the two sides together, Joe Allison is shot and Fred blamed. With Fred in jail and a lynch mob on the way, Doc tries to break his friend out.
Bob Blake and his sidekick and four singing cowboys arrive at the Jackson ranch where Bob learns from Betty Jackson that her brother, Joe, is missing. Bob investigates and learns that there is gold on the Jackson ranch, and the neighboring rancher has kidnapped Joe in order to get his land.
Gene takes care of three tough kids sent west from Chicago after their father died and left them a cattle ranch. They help him catch a bunch of rustlers.
In his starring debut, Roy gets elected to Congress in order to bring water to the ranchers in his district. In Washington, he learns he needs the backing of a key congressman and gets that man to go west for an inspection trip. When the congressman is initially unimpressed, Roy gets the inspection party stranded without water to show the true conditions.
Saunders with his Cattlemen's Protective Agency is running roughshod over the ranchers. Lawyer Larry Kimball is fighting him but he needs a rancher that will stand up with him against Saunders. He finds him when Lou Gehrig retires from baseball to take up ranching. Lou expects to relax on his ranch but quickly joins Larry in the fight.
Dare Rudd takes a shine to his cattleman cousin Tom's girlfriend who asks Tom to hire Dare to head the big cattle drive. Dare loses the money for the drive to cardsharps, but Tom wins it back, but Dare must save Tom's life.
Ranch owner Sandra, fresh from animal husbandry school, brings a flock of sheep into cattle country. The local ranchers don't like it, and ranch foreman Gene must deal with it.