A group of people traveling on a stagecoach find their journey complicated by the threat of Geronimo, and learn something about each other in the process.
The Colonel sends Fred Dawson and Doc Flanders to investigate a cattleman sheepman war. Posing as a two man medicine show, they quickly become involved. When Fred tries to bring the two sides together, Joe Allison is shot and Fred blamed. With Fred in jail and a lynch mob on the way, Doc tries to break his friend out.
When shifty cattlemen Belknap (Walter Miller) and H.R. Shelby (Gordon Hart) are caught shipping infected animals to Mexico, they frame inspector Gene Autry. Now Autry and his sidekick, Frog Millhouse (Smiley Burnette), must catch the bad guys in the act and set things straight. June Storey co-stars as rancher Martha Wheeler. Autry sings "I'm Gonna Round Up My Blues," "Moonlight on the Ranch House" and "Big Bull Frog."
Bob Blake and his sidekick and four singing cowboys arrive at the Jackson ranch where Bob learns from Betty Jackson that her brother, Joe, is missing. Bob investigates and learns that there is gold on the Jackson ranch, and the neighboring rancher has kidnapped Joe in order to get his land.
Knowing that is contains valuable helium gas, a gang of bad guys first tries to purchase the ranch which Gene straw-bosses. When that fails, they lay a hidden pipeline to snag the gas.
Gene takes care of three tough kids sent west from Chicago after their father died and left them a cattle ranch. They help him catch a bunch of rustlers.
Scanlon is pulling off a land swindle by selling lots in a ghost town claiming the power company is bringing in a line. As a bonus he throws in shares in a worthless gold mine. Gene is on to Scanlon and tries to get him to buy back the deeds by salting the mine with gold. But when a new vein is really discovered Gene has to stop the sales but is trapped in the mine by Scanlon's men.
In his starring debut, Roy gets elected to Congress in order to bring water to the ranchers in his district. In Washington, he learns he needs the backing of a key congressman and gets that man to go west for an inspection trip. When the congressman is initially unimpressed, Roy gets the inspection party stranded without water to show the true conditions.
Saunders with his Cattlemen's Protective Agency is running roughshod over the ranchers. Lawyer Larry Kimball is fighting him but he needs a rancher that will stand up with him against Saunders. He finds him when Lou Gehrig retires from baseball to take up ranching. Lou expects to relax on his ranch but quickly joins Larry in the fight.
Dare Rudd takes a shine to his cattleman cousin Tom's girlfriend who asks Tom to hire Dare to head the big cattle drive. Dare loses the money for the drive to cardsharps, but Tom wins it back, but Dare must save Tom's life.
Ranch owner Sandra, fresh from animal husbandry school, brings a flock of sheep into cattle country. The local ranchers don't like it, and ranch foreman Gene must deal with it.
Young Englishman inherits ranch which he wants to sell, but Gene's gonna turn him into a real westerner instead. When new owner Spud arrives from England, Autry convinces him not to sell the ranch but to raise horses for the Army. When both Autry's and Neale's bids are the same, the Colonel calls for a race to decide the winner. But that night Neale has Autry's stable burned.