Hoppy, California and Lucky take refuge from a storm inside a supposedly abandoned church outside a ghost town, only to meet a young woman and her mother there, then find themselves surrounded by a gang of "workmen" intent on tearing down the church if they have to kill the five to do it.
Based on and built around the west coast radio program, "The Hollywood Barn Dance", although no members of the 1947 cast of the program are in the film, but the better-known (on a national scale) Ernest Tubb and His Texas Troubadors, Jack Guthrie and Jimmy and Leon Short more than make up for that. The slight plot, around 18 songs, begins with Tubb and his band searching for $2000 needed to rebuild their town chuch after it burned down while they were rehearsing in it. Hollywood, here they come!
Autry is drawn into the plot when he's given power of attorney in a property settlement involving his old pal and a gang of land swindlers. The pal then goes on an extended vacation, leaving Autry to sort things out.
Marvin Hayden returns to find his ranch is about to be sold at auction and the Hayden Jorth feud still going strong. Carson wants the Hayden ranch and tries to kill Hayden. When he fails he kills Chick Jorth with a rock. As Hayden does not carry a gun and the two had argued earlier, Hayden is arrested for the murder. With Hayden in jail, his friends Chito, Ginger, and his Lawyer Gardner now go to work to find the murderer.
Red Ryder convinces homesteaders to settle in Paradise Valley. Business men in nearby Central City want control of the valley and water supply and propose to build a dam for half interest in the land. They use Red to generate interest in the dam but when the dam is completed, they rig the stockholder's meeting so Central City will get the water.
A boy haunted by nightmares about the night his entire family was murdered is brought up by a neighboring family in the 1880s. He falls for his lovely adoptive sister but his nasty adoptive brother and mysterious uncle want him dead.
A famous bandleader, suffering from overwork and exhaustion, goes to a sanitarium for a rest. While there he dreams of being out west at a dude ranch, where he finds himself involved in the beautiful owner's struggle to keep her ranch from falling into the hands of the villain, who wants either her or her ranch (or, preferably, both).
Knowing the railroad is coming, Carter is after the rancher's land. Bob and Chito return just in time to save Banker Stockton and his money from Carter's men. When Stockton then lends the ranchers money, Carter has them burned out. Bob knows Carter is responsible and when Carter's henchman Saunders is recognized, Bob goes into action.
Roy is an oil prospector. His efforts to get drilling rights on an old Spanish land grant are countered by gamblers from an off-shore gambling boat determined to control the land (and oil) themselves.
Notorious shootist and womanizer Quirt Evans' horse collapses as he passes a Quaker family's home. Quirt has been wounded, and the kindly family takes him in to nurse him back to health against the advice of others. The handsome Evans quickly attracts the affections of their beautiful daughter, Penelope. He develops an affection for the family and their faith, but his troubled past follows him.
Miss Hinklefink inherits a western ranch, and to spend the summer with Professor Townley, she invites Freddie, Dodie, Betty, Lee, and Roy to spend their vacation on the ranch if Townley will chaperone. Meanwhile, a real estate agent tries to persuade her to send the kids home when desperadoes rob the bank.
Gene Autry is back near the saddle, trying to help out a crippled jockey. Gene is certain that the jockey can ride in the Big Race if the lad can regain his self-confidence. Meanwhile, Gene and comical sidekick Sterling Holloway have another problem on their hands: A rogue stallion has "kidnapped" Gene's prize mare. Piloting a plane, Autry seeks out and locates the stallion.
Caxton has broken out of prison and Eddie has been sent to bring him in. Caxton is known by the polka dot band on his hat and Eddie has Soapy wear one like it. This gets Soapy arrested as soon as he rides into town but it leads Eddie to Varney and he realizes Varney will lead him to Caxton.
Chasing women and staying one step ahead of the law, the Cisco Kid meets Raquel and then Dolores. He sees that Raole is the boy friend of Raquel but engaged to Dolores. Learning that all her money will got to her uncle Don Jose when she marries Raole, Cisco suspects a plot and sets out to unravel it.
Lambert has the stagecoach wrecked killing the Commissioner so his phony replacement can alter Coonskin's land survey. When Red Ryder exposes the survey hoax, Lambert has his stooge Sheriff put Red in jail.