Young Joe is paralyzed as he is bucked by a wild horse, a strawberry roan. Angered, his father, Walt, tries to shoot the horse but is stopped by his foreman, Gene Autry. The roan escapes and Autry, told to leave the ranch by Walt, finds and trains the horse, now named Champ, in hopes that by returning it to Joe it will provide him with the will to overcome his disability.
A ranch owner turns his place into a home for boys who have lost their fathers in World War II. His evil female lawyer covets the ranch and uses a gang of local toughs, a pack of killer dogs, and a phoney rancher's beneficiary to get it. U.S. Marshal Rogers opens an investigation when the rancher is killed.
James Craig is torn between his criminal career as the masked bandit named the "El Paso Kid," and the life of a law-abiding citizen with his long-suffering wife Zoe. He repeatedly tells Zoe, "just one more time," but he is unable to stop which angers her greatly. However, he does have brief moments of heroics such as when he helps the Widow Weeks save her farm.
When the bank is robbed, Gene and the boys are singing nearby and the Chief arrests them as gang members but lets them go thinking they will lead them to the others.
Jean Loring has her men illegally killing and selling game. Roy suspects her and gets himself invited to stay at her ranch. Investigating he finds the freezer where the slaughtered game are kept. But he is caught, tied up, and left to freeze.
Based on and built around the west coast radio program, "The Hollywood Barn Dance", although no members of the 1947 cast of the program are in the film, but the better-known (on a national scale) Ernest Tubb and His Texas Troubadors, Jack Guthrie and Jimmy and Leon Short more than make up for that. The slight plot, around 18 songs, begins with Tubb and his band searching for $2000 needed to rebuild their town chuch after it burned down while they were rehearsing in it. Hollywood, here they come!
Autry is drawn into the plot when he's given power of attorney in a property settlement involving his old pal and a gang of land swindlers. The pal then goes on an extended vacation, leaving Autry to sort things out.
Gridley is mining silver from an old Mexican mine and bringing it into the USA thru a passage into his worthless mine. Border guard Rogers suspects Gridley and finally finds the secret entrance to the Mexican mine. He sends Lee Madison for help only to have her captured by Gridley. Trigger brings help that takes care of Gridley's men and now Roy has to rescue Madison.
Red Ryder convinces homesteaders to settle in Paradise Valley. Business men in nearby Central City want control of the valley and water supply and propose to build a dam for half interest in the land. They use Red to generate interest in the dam but when the dam is completed, they rig the stockholder's meeting so Central City will get the water.
When Decker's gang holds up a stage, henchman Lefty takes a lady's rings. Later lefty accidentally exposes the rings buying ammunition and Cheyenne sees them. When Lefty tries to shoot Cheyenne he is captured. Now Cheyenne wants the rest of the gang and their leader.
Knowing the railroad is coming, Carter is after the rancher's land. Bob and Chito return just in time to save Banker Stockton and his money from Carter's men. When Stockton then lends the ranchers money, Carter has them burned out. Bob knows Carter is responsible and when Carter's henchman Saunders is recognized, Bob goes into action.
Notorious shootist and womanizer Quirt Evans' horse collapses as he passes a Quaker family's home. Quirt has been wounded, and the kindly family takes him in to nurse him back to health against the advice of others. The handsome Evans quickly attracts the affections of their beautiful daughter, Penelope. He develops an affection for the family and their faith, but his troubled past follows him.
Gene Autry is back near the saddle, trying to help out a crippled jockey. Gene is certain that the jockey can ride in the Big Race if the lad can regain his self-confidence. Meanwhile, Gene and comical sidekick Sterling Holloway have another problem on their hands: A rogue stallion has "kidnapped" Gene's prize mare. Piloting a plane, Autry seeks out and locates the stallion.
Lambert has the stagecoach wrecked killing the Commissioner so his phony replacement can alter Coonskin's land survey. When Red Ryder exposes the survey hoax, Lambert has his stooge Sheriff put Red in jail.