Rancher Autry takes a job singing on the radio to aid farmers and ranchers whose lands were destroyed by raging floods. Blaming crooked politicians, he goes to Washington and tries to put through a food control bill and finds he has a lot to learn. In this classic release, Gene introduces his immortal theme song, "Back in the Saddle Again," which has gone on to become a piece of American History.
Ann Fenwick is a witness to a bank robbery in the U.S. and the bandits, led by Trigger and Leon capture her and when she disappears, a warrant is issued for her arrest as a material witness. The bank robbers flee across the border into Canada where they steal a trailer in which they lock Ann and the loot. The hitch breaks and the trailer plunges into a lake. Sergeant Renfrew and Constable Kelly, of the Canadian Mounties, rescue Ann and she tells them she is a hitch-hiking tourist and gives a false name. Renfrew sends Kelly for aid, Ann escapes and Kelly returns with the news that she is wanted. The leader of the gang, Cardigan, sends the gang back for Ann and the loot, which Ann has hidden in a trappers cabin, just before Trigger recaptures her. Renfrew goes to her rescue, but is also captured. But reliable Constable Kelly is somewhere in the woods.
Before he was killed, Martin hid a half million dollars worth of bonds on his ranch. Brainard, who killed him, Inspector Carson posing as Sam Brown, and Martin's niece Margaret all want the ranch, and it's being sold at auction.
When his ranch falls on hard times, Cowboy Roy Roger has trouble making his mortgage payment and he takes his song and dance to Wall Street to try to raise cash fast.
When his well-meaning sidekick (Smiley Burnette) buys a cow farm instead of a cattle ranch, singing cowpoke Gene Autry prepares to embrace the dairy business. But with a corrupt association bent on driving up milk prices, it's up to newly elected Sheriff Gene to clean up the mess. Country music icon Patsy Montana sings "I Want to Be a Cowboy's Sweetheart," while radio crooners the Texas Rangers perform alongside Autry.
Cavanaugh and McCauley are after the ranchers land. When the Government announces the land will be put up for auction, the ranchers pool their money only to have it stolen by Cavanaugh's men. They then plan to sell their cattle but Cavanaugh announces a fake gold strike and the cowhands all leave. But Gene's hobo friend the Judge says he will get the cattle to market and he sends out a signal to his hobo friends.
In the midst of the Civil War, Lassiter has a plan to get control of California. Working out of St. Joseph, he plans to send forged messages to the troops on the west coast via Pony Express. First he attempts to bribe Pony Express ride Roy Rogers. When Roy refuses he turns to the outlaw Johnson and his gang and this leads to trouble.
Roy and Gabby return to Gabby's Texas ranch, after fighting with the Confederate military during the American Civil War, to find that a blustery Union Colonel whom they have previously hassled is now their district commander. Unbeknownst to the Colonel, however, is that the soldiers he believes have been sent to assist him are actually Union Army rejects who have come to loot the civilian populace under the guise of reinstituting normalcy to the former Confederate district.
Roy Rogers is a cowboy who joins the Border Patrol, only to have his buddy Tommy get killed at a local saloon. Determined to get revenge at any cost, Roy and Rusty cross the border in search of Arizona Jack, the man responsible for Tommy's death.
A group of people traveling on a stagecoach find their journey complicated by the threat of Geronimo, and learn something about each other in the process.
When Blackton outbids Bill Carson. Bill suspects he will have to rustle cattle to fulfill the contract. So Bill arrives posing as an Mexican. When he rustles the cattle from the rustlers, it gets him into the gang. Hoping to bring them all to justice, he is in trouble when his true identity is revealed.
When shifty cattlemen Belknap (Walter Miller) and H.R. Shelby (Gordon Hart) are caught shipping infected animals to Mexico, they frame inspector Gene Autry. Now Autry and his sidekick, Frog Millhouse (Smiley Burnette), must catch the bad guys in the act and set things straight. June Storey co-stars as rancher Martha Wheeler. Autry sings "I'm Gonna Round Up My Blues," "Moonlight on the Ranch House" and "Big Bull Frog."
Knowing that is contains valuable helium gas, a gang of bad guys first tries to purchase the ranch which Gene straw-bosses. When that fails, they lay a hidden pipeline to snag the gas.
Mary Smith decides after a lifetime of being a shut-in to do something wild while her father is out campaigning for the presidency, so she takes off for the family's home in West Palm Beach and inadvertently becomes romantically entangled with earnest cowboy Stretch Willoughby. Neither the dalliance nor the cowboy fit with the upper class image projected by her esteemed father, forcing her to choose.