In pre-Revolutionary America, the efforts of a Colonial officer trying to broker a peace deal between Indian chief Pontiac and British and American settlers are threatened by the commander of a Hessian mercenary unit who embarks on a campaign of extermination against the Indians.
Jerry Johnson inherits a 50,000 acre ranch. Lucky Miller wants to take over the ranch. Roy is trying to get a railroad spur right of way. Lucky has a woman come west to marry Jerry to get control of the ranch. After the wedding, Lucky has the owner killed. Roy’s gun is substituted for the murder weapon, so Roy is put in jail.
In the midst of the Civil War, Lassiter has a plan to get control of California. Working out of St. Joseph, he plans to send forged messages to the troops on the west coast via Pony Express. First he attempts to bribe Pony Express ride Roy Rogers. When Roy refuses he turns to the outlaw Johnson and his gang and this leads to trouble.
Seeker Dean has found the gold he has been looking for for 15 years. Heading for the Government office, Boone Jackson kills him. Kickabout finds a cryptogram as to the gold's location and Sergeant Kinkaid solves the puzzle. But Jackson learns of the gold's location and to get it, he sets out to dynamite the dam that would flood the entire communuty.
Roy returns home to fine a range feud between the cattlemen and the sheepmen. When his friend is killed he finds the rifle had a defective pin. He learns the rifle belongs to a ranch hand named Barker and that a third party has caused the feud. When he captures outlaws trying to blow up a dam, he claims Barker was the killer. But Barker has switched rifles and the outlaws now accuse Roy and Roy finds himself in trouble.
Finding Indians stealing from his ranch, Gene learns they are suffering from malnutrition. Store owner Martin is cheating them and now he is after the Chief's valuable necklace. When the dying chief is found, having been attacked and robbed, Martin blames Lakhona who would become the new chief. When Gene helps Lakhona they soon find themselves fleeing from the law.
In Old California, a young Frenchman transporting a chest full of silver travels by stagecoach to San Marino, to complete a complex business deal. The stagecoach is ambushed by a band of men whose leader, a mysterious bandido known as Cisco (Gilbert Roland), claims the silver is money that was extorted over a period of years from the poor people of California. The bandits take the money and escape, but Cisco stays behind with the Frenchman -- who, it turns out, is actually a lovely mademoiselle, Jeanne DuBois (Ramsay Ames). She follows him to the bandit's lair, where Cisco tells her he intends to return the stolen money to the poor people. The two rivals are irresistibly drawn to each other, however, and as a token of love Cisco offers to return the money to Jeanne instead. Now she must decide whether to complete her business deal, or to comply with Cisco's wishes and redistribute the wealth.
Rancher Reynolds has fired his men and hired killers and is now using a crooked land deal to put the other ranchers off their land. Calico finds the reason why when he runs into his old nemesis Porter.
Jeff arrives in town to see the Sheriff only to find him just killed. The culprit is Clay Wheeler. When Jeff becomes friendly with Letty, Clay sends his man Ortega to kill him. Jeff foils the attempt and gets him to confess that Clay was the killer. With only old-timers Lafe and Bill to help, Jeff heads after Clay and his gang.
When the showboat hits town, two men use the parade as a distraction to rob the bank. Their accomplice is Pop, the clown from the showboat. He leaves the money on the boat and tells his daughter Patsy to bring it to him at a later stop on the river. Gene's investigation of a bank robbery takes him to the showboat where he becomes a performer. Gene and Frog try to find the money while helping Patsy and her father.
Poachers are harassing toll road owner Jen Larrabee. They want her land because it holds valuable minerals. Autry and the Cass County Boys, mistaken for Texas Rangers, help out.
Deputies Gene Autry and Frog go up against modern cattle rustlers. These rustlers use technology such as, airplanes, radios and refrigerated trucks to steal the cows, butcher them in the field and ship them out before getting caught. This causes the town to bring in a modern NYC detective to catch the crooks, but will Autry and Frog be permanently out of a job?
Jean Loring has her men illegally killing and selling game. Roy suspects her and gets himself invited to stay at her ranch. Investigating he finds the freezer where the slaughtered game are kept. But he is caught, tied up, and left to freeze.
20 years ago, 3 men robbed a stage and hid $30,000. They were caught and sent to prison by Marshal Steve Autry. 20 years later, the men bust out of prison and return to the ghost town where they stashed their treasure searching. Steve's grandson picks up where Steve left off to foil the plans of the outlaws.
Safely from behind some shrubbery, Johnny Hume, a boy of 6 or 7, witnesses the slaughter of his mother, father and brother by the guns of a gang led by "the Cat". Twenty years later finds Johnny grown to manhood, an expert bronc rider and target shooter - but paralyzed with fearful memories in an actual gunfight. This is brought home to him when some outlaws stick up the local saloon and Johnny ends up cowering behind the bar.