Trouble in Colorado is tying up Union troops needed back east during the Civil War and Lieut. Burke is sent to investigate. Macklin and his gang are causing the problems and Capt. Mason joins them. When Burke catches up with them he also finds Mason, his brother.
Julie's husband has been murdered and land agents want her to sign away her property rights. Hoppy warns against this but she does so anyway. It looks as though she will be unable to deliver the timber called for in her agreement. Hoppy has to make the lumber deal happened and solve the murder.
Mountie Matt O'Brien is assigned to escort Miss Owens to a remote outpost. But when he finds an illegal mining operation there that is smuggling gold across the border, his superior Sgt. Means orders him to leave.
Roy returns home to fine a range feud between the cattlemen and the sheepmen. When his friend is killed he finds the rifle had a defective pin. He learns the rifle belongs to a ranch hand named Barker and that a third party has caused the feud. When he captures outlaws trying to blow up a dam, he claims Barker was the killer. But Barker has switched rifles and the outlaws now accuse Roy and Roy finds himself in trouble.
Jeff arrives in town to see the Sheriff only to find him just killed. The culprit is Clay Wheeler. When Jeff becomes friendly with Letty, Clay sends his man Ortega to kill him. Jeff foils the attempt and gets him to confess that Clay was the killer. With only old-timers Lafe and Bill to help, Jeff heads after Clay and his gang.
When the showboat hits town, two men use the parade as a distraction to rob the bank. Their accomplice is Pop, the clown from the showboat. He leaves the money on the boat and tells his daughter Patsy to bring it to him at a later stop on the river. Gene's investigation of a bank robbery takes him to the showboat where he becomes a performer. Gene and Frog try to find the money while helping Patsy and her father.
Deputies Gene Autry and Frog go up against modern cattle rustlers. These rustlers use technology such as, airplanes, radios and refrigerated trucks to steal the cows, butcher them in the field and ship them out before getting caught. This causes the town to bring in a modern NYC detective to catch the crooks, but will Autry and Frog be permanently out of a job?
Jean Loring has her men illegally killing and selling game. Roy suspects her and gets himself invited to stay at her ranch. Investigating he finds the freezer where the slaughtered game are kept. But he is caught, tied up, and left to freeze.
Cholita, after a long absence in Mexico City, is returning home to take up her duties as head of the rancho and, as everyone expects, to marry her childhood sweetheart José. Expectations are somewhat dashed as she shows up with Fernando to whom she is engaged. This makes José and Cholita's uncle more than a little bit put out as Fernando is not only not a Mexican, he is also a city slicker afraid of the country.
Americans come west to California in the hope of peaceful settlement. Roy and Gabby sing a duet: "We're Not Coming Out Tonight." Other songs include "Sundown on the Rangeland" and "Ride on Vaquero."
The Carson City Kid and partner Laramie are outlaws. When his partner is caught the Kid, his identity being unknown, takes a job in Jessup's saloon. Here he see Jessup cheat Waren out of his money. Warren then robs Jessup posing as the Kid but gets caught. To gain his freedom, Laramie identifies Warren as the Kid. Realizing Jessup is the man that killed his brother, the Kid must find a way to clear Warren and get Jessup.
Wanted by the law in New York, Dr. Steve Kells heads west and arrives in an area controlled by an outlaw gang known as the Border Legion. When the gang's boss is wounded, they kidnap Kells and force him to remove the bullet. Not allowed to leave and being a wanted man, he joins the gang. Now wanted as a gang member also, he nevertheless plans a raid that will lead the entire gang into a trap.
Sandwiched in between the numerous musical numbers, the Gabby Whittaker and Madden rodeo's are competing for bookings. When Gabby gets a date in Albuquerque, Madden has his man destroy his equipment. Roy finds a broken rawhide rope at the scene and uses it to bring Madden to justice.