Gene Autry hunts bank robbers Al Bartlett and Trot Lucas with his old friend Mike. Bartlett, to throw off his pursuers, kills Trot and his own brother. When Kitty Bartlett comes to town claiming to be the slain Bartlett's widow, Gene has to save her from the irate townspeople who are not aware that her name isn't Bartlett but she really is the daughter of a law officer slain by Al Bartlett. Ben Luder, a local hood, tricks Bartlett back into town by saying he has to fixed to have Doc Larry Taylor do plastic surgery on him. En route they meet Doc and his assistant Helen Ellis and Ben's ruse is exposed. Bartlett kills Ben and forces Doc to drive him to the railroad. Gene, in a fight atop a runaway train, captures Bartlett.
Gene Autry becomes the new Sheriff after bank robbers kill the former sheriff. When Judge Beaumont is murdered, evidence points to the judge's wild son. Believing the young man, Gene tries to help.
Montana ranch owner Cyrus Bigbee sends his foreman, Gene Autry, and Rawhide Buttram to his Canadian timber land to stop the marriage of his daughter Sandy to Todd Markey, whom he dislikes. Sandy wants to turn the property into a dude ranch, with Carolina Cotton and the Cass County Boys (Fred S. Martin, Jerry Scoggins and Bert Dodson) among the entertainers, and runs up against local timbermen who want it for cutting timber. When a Mountie is murdered, with suspicion pointing to Todd, Gene finds the real culprit and brings peace to the area.