Two celebrated gunfighters are separately hired to come to a small Canadian town in Manitoba named "Glory" for a showdown at high noon. But before they can do that, they join forces and take on a bandit gang that dominates the area.
Pete Marlan is a gambler with an unsavory past. Suspected of being an outlaw, Pete plays along with this misconception, the better to infiltrate a gang of smugglers. Along the way, he clears the name of the brother of Texas Ranger Mike Donovan, and helps patch up the romance between Donovan and heroine Bernice.
Marshals Nevada and Sandy are after Scully and his gang who have been robbing stage-coaches. The Texas Kid is part of the gang and Sandy thinks he is bad but Nevada knows him and thinks he may be good.
A landslide has diverted water from the Baldwin ranch to Cambert's. With their cattle dying, Cambert refuses to let them have any water. Easterner Larry Knight takes a job with the Baldwins and he has a plan to divert the water back to the Baldwin ranch. But Phil, jealous of Kitty's attraction to Larry, lets Cambert know of the scheme.
A crusading newspaper editor recruits his old friend Hoppy to take the job of Marshall in a town rife with vice and murder directed at helpless miners.
A gunslinger on the run for a murder he didn't commit goes to New Mexico where he hopes to find a person who can exculpate him. While on his way he finds a girl who has been kidnapped and tortured, sets her free and then takes his revenge at gunpoint.
Banker Mason is after the ranchers land so he can resell it to the railroad for a profit. He has the railroad agent killed and replaces him with his stooge who then offers even less than Mason. But Rocky eventually suspects Mason and when Bill Anderson informs him the agent is a fake, they head out after Mason
Gene returns from the East with new ranch owner Tom Bennett to find everyone's cattle dying. Blaine has reopened the copper mine and the waste is poisoning the water supply. While Gene is away Tom confronts the miners and a man is killed in the ensuing gunfight. Now Gene not only has the dying cattle problem but his ranch owner is in jail.
In this Roy Rogers entry, featuring a song written by Oklahoma Governor Roy J. Turner (making him and Lousiania's Jimmie Davis and Texas' W.E. "Pappy" O'Daniel possibly the only state governors to write songs used in a western), Flying U ranch owner Sam Talbot is killed by a fall from a horse. St. Louis reporter Connie Edwards comes to check a rumor that he might have been murdered. She goes to Roy Rogers, editor of the local newspaper, and he takes her to the reading of Talbot's will. The ranch is left to Talbot's 12-year-old ward, Duke Lowery, much to the dismay of Talbot's niece, Jan Holloway. After some attempts on Duke's life, Roy finally proves that Jan, Steve McClory and coroner Jim Judnick had Talbot killed and are conspiring to do the same for Duke, making Jan the last heir.
Rancher Tim Clark borrows money from Bob Russell, who then rustles Clark's cattle so he will be unable to repay the money. Thus Russell is able to cheat Clark out of his ranch. Clark becomes a prospector for silver and ultimately comes to settle accounts with Russell and crooked deputy Bendix.
In the midst of the Civil War, Lassiter has a plan to get control of California. Working out of St. Joseph, he plans to send forged messages to the troops on the west coast via Pony Express. First he attempts to bribe Pony Express ride Roy Rogers. When Roy refuses he turns to the outlaw Johnson and his gang and this leads to trouble.
Molly, the shy, romance-starved wife of an arrogant frontier sheriff, finds herself drawn to a prisoner in her husband's jail. This prisoner, a handsome young man named Johnny, plays on Molly's sympathy and convinces her to help him escape. Molly then accompanies Johnny on his cross-country flight but soon learns he's simply been using her. Molly makes the best of the situation, however, and by the time the sheriff's posse catches up with them, Molly shows that she's learned how to assert herself.